Expression of accumulated capacity for initiation of chromosome and minichromosome replication in dnaA mutants of Escherichia coli.

AUTOR(ES)
RESUMO

Chromosome and minichromosome replication were examined in temperature-sensitive dnaA mutants of Escherichia coli growing at temperatures between permissive and nonpermissive. Periodicities in [14C]thymidine uptake were detected as cultures incubated at intermediate temperatures approached late exponential-early stationary phase of growth. Exposure of the cultures to a nutritional shift-up caused a stimulation of chromosome replication associated with a rapid initiation of new rounds of replication, very similar to that observed after exposure to chloramphenicol. Addition of rifampin also caused a stimulation, but to a much lesser extent. The induced initiations of chromosome replication took place in two waves, as was the case when the cultures were simply shifted to permissive temperature. Minichromosomes were also stimulated to replicate by the addition of chloramphenicol at intermediate temperatures, providing further evidence that the chromosomal region which responded to the chloramphenicol treatment was in the vicinity of oriC. The findings are consistent with the conclusion that the initiations induced by chloramphenicol are consequences of the involvement of the dnaA gene product in a transcriptional step at initiation, as suggested by Orr et al. The results also suggest that the activity of the dnaA gene product is not normally involved in controlling the frequency of initiation of chromosome replication.

ACESSO AO ARTIGO

Documentos Relacionados