Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein.

AUTOR(ES)

Ostermayr, R

RESUMO

The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with rabbit anti-HAV serum, suggesting that it possesses HAV antigenicity.

Documentos Relacionados

• Expression of the polyomavirus minor capsid proteins VP2 and VP3 in Escherichia coli: in vitro interactions with recombinant VP1 capsomeres.
• Hepatitis A Virus Capsid Protein VP1 Has a Heterogeneous C Terminus
• Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
• Molecular cloning of the cowpea leghemoglobin II gene and expression of its cDNA in Escherichia coli. Purification and characterization of the recombinant protein.
• Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application