Expression of the cloned ColE1 kil gene in normal and Kilr Escherichia coli.
AUTOR(ES)
Altieri, M
RESUMO
The kil gene of the ColE1 plasmid was cloned under control of the lac promoter. Its expression under this promoter gave rise to the same pattern of bacterial cell damage and lethality as that which accompanies induction of the kil gene in the colicin operon by mitomycin C. This confirms that cell damage after induction is solely due to expression of kil and is independent of the cea or imm gene products. Escherichia coli derivatives resistant to the lethal effects of kil gene expression under either the normal or the lac promoter were isolated and found to fall into several classes, some of which were altered in sensitivity to agents that affect the bacterial envelope.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213530Documentos Relacionados
- Expression of the kil gene of the ColE1 plasmid in Escherichia coli Kilr mutants causes release of periplasmic enzymes and of colicin without cell death.
- RNase H and replication of ColE1 DNA in Escherichia coli.
- Mitomycin-induced lethality of Escherichia coli cells containing the ColE1 Plasmid: involvement of the kil gene.
- cea-kil operon of the ColE1 plasmid.
- Suppression of ColE1 high-copy-number mutants by mutations in the polA gene of Escherichia coli.
