Expression of the cloned uracil permease gene of Saccharomyces cerevisiae in a heterologous membrane.
AUTOR(ES)
Chevallier, M R
RESUMO
A piece of DNA of the yeast Saccharomyces cerevisiae complementing the uracil permease gene was introduced into a plasmid able to replicate autonomously in Schizosaccharomyces pombe. A strain of S. pombe lacking uracil transport activity was transformed with this new plasmid carrying the gene of S. cerevisiae. The behaviour of the transformant shows not only an expression of the uracil permease gene in the heterologous membrane but also that the transport of uracil is active and coupled to the energy furnishing system of the heterologous host.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553052Documentos Relacionados
- Homologous versus heterologous gene expression in the yeast, Saccharomyces cerevisiae.
- Upstream region of the SUC2 gene confers regulated expression to a heterologous gene in Saccharomyces cerevisiae.
- The phosphoinositol sphingolipids of Saccharomyces cerevisiae are highly localized in the plasma membrane.
- GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae.
- Enhanced Iron Uptake of Saccharomyces cerevisiae by Heterologous Expression of a Tadpole Ferritin Gene
