Expression of the Hantaan virus M genome segment by using a vaccinia virus recombinant.
AUTOR(ES)
Pensiero, M N
RESUMO
A cDNA containing the complete open reading frame of the Hantaan virus (HTN) M genome segment has been cloned into vaccinia virus. This recombinant virus expresses two glycoproteins which are similar to the HTN structural glycoproteins, G1 and G2, in molecular weight, cleavage pattern, and cellular distribution. Both HTN and recombinant vaccinia virus glycoproteins are exclusively associated with the Golgi apparatus of the cell. Despite this intracellular restriction, mice inoculated with the recombinant vaccinia virus raised neutralizing antibodies against HTN. The specificity of virus neutralization appears to reside in the HTN glycoproteins, since a vaccinia virus recombinant expressing the HTN nucleocapsid protein was unable to elicit a neutralizing antibody response.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=253622Documentos Relacionados
- Rescue of Sindbis virus-specific RNA replication and transcription by using a vaccinia virus recombinant.
- Nucleotide sequence of the L genome segment of Hantaan virus.
- Rift Valley fever virus M segment: use of recombinant vaccinia viruses to study Phlebovirus gene expression.
- Nucleotide sequence of the M segment of the genomic RNA of Hantaan virus 76-118.
- Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants.