Expression of Ty-lacZ fusions in Saccharomyces cerevisiae.
AUTOR(ES)
Bowen, B A
RESUMO
We have determined the nucleotide sequence of about 520 bp spanning the 5' delta regions (Figure 1) of two Tyl elements. There is an open reading frame running out of the deltas for at least 180 nucleotides into the internal region of each element. The functional significance of these open reading frames has been tested by fusing them to a defective E.coli lacZ gene. Expression of B-galactosidase in yeast transformants containing these fusions shows that Tyl elements contain functional translation signals.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318604Documentos Relacionados
- Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.
- Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
- Genetic mapping of Ty elements in Saccharomyces cerevisiae.
- Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.
- Regulation of RAD54- and RAD52-lacZ gene fusions in Saccharomyces cerevisiae in response to DNA damage.
