Fate of Transforming Deoxyribonucleic Acid After Uptake by Competent Bacillus subtilis: Size and Distribution of the Integrated Donor Segments

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RESUMO

A series of sheared donor-recipient complex samples were prepared from a competent Bacillus subtilis culture which had been transformed by using 3H, 2H-labeled deoxyribonucleic acid. Measurement of the molecular weights and buoyant densities of these samples led to the following conclusions. (i) The average size of the single-stranded donor segment which is integrated is 2.8 × 106. (ii) These donor segments are not randomly distributed in the recipient genome, but are clustered in groups, each group containing several discrete donor segments.

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