Fermentation of Fructose and Synthesis of Acetate from Carbon Dioxide by Clostridium formicoaceticum1
AUTOR(ES)
O'Brien, W. E.
RESUMO
Clostridium formicoaceticum ferments fructose labeled with 14C in carbon 1, 4, 5, or 6 via the Embden Meyerhof pathway. In fermentations of fructose in the presence of 14CO2, acetate is formed labeled equally in both carbons. Extracts convert the methyl groups of 5-methyltetrahydrofolate and methyl-B12 to the methyl group of acetate in the presence of pyruvate. Formate dehydrogenase, 10-formyltetrahydrofolate synthetase, 5,10-methenyltetrahydrofolate cyclohydrolase, 5,10-methylenetetrahydrofolate dehydrogenase, and 5,10-methylenetetrahydrofolate reductase are present in extracts of C. formicoaceticum. These enzymes are needed for the conversion of CO2 to 5-methyltetrahydrofolate. It is proposed that acetate is totally synthesized from CO2 via the reactions catalyzed by the enzymes listed above and that 5-methyltetra-hydrofolate and a methylcorrinoid are intermediates in this synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=285186Documentos Relacionados
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