Fertile and diploid nuclear transplants derived from embryonic cells of a small laboratory fish, medaka (Oryzias latipes)

AUTOR(ES)

Wakamatsu, Yuko

FONTE

The National Academy of Sciences

RESUMO

Fertile and diploid nuclear transplants were successfully generated by using embryonic cells as donors in a small laboratory fish, medaka (Oryzias latipes). Embryonic cell nuclei from transgenic fish carrying the green fluorescent protein (GFP) gene were transplanted into unfertilized eggs enucleated by x-ray irradiation. In this study, 1 out of 588 eggs transplanted in the first experiment and 5 out of 298 eggs transplanted in the second experiment reached the adult stage. All of these nuclear transplants were fertile and diploid, and the natural and GFP markers of the donor nuclei were transmitted to the F1 and F2 offspring in a Mendelian fashion. This systematic study proves the feasibility of generating nuclear transplants by using embryonic cells from fish as donors, and it is supported by convincing evidence.

Documentos Relacionados

• Development of a possible nonmammalian test system for radiation-induced germ-cell mutagenesis using a fish, the Japanese medaka (Oryzias latipes).
• DNA alterations detected in the progeny of paternally irradiated Japanese medaka fish (Oryzias latipes).
• Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning
• A Further Study on Induction of Functional Sex Reversal in Genotypic Males of the Medaka (Oryzias Latipes) and Progenies of Sex Reversals
• Progeny of Artificially Induced Sex-Reversals of Male Genotype (Xy) in the Medaka (Oryzias Latipes) with Special Reference to Yy-Male