Fertility inhibition gene of plasmid R100.
AUTOR(ES)
McIntire, S A
RESUMO
The fin0 gene of R100 was isolated from the Fin0+ transducing phage VA lambda 57. The limits of the gene were determined by BAL31 digestions and by analysis of deletion mutations derived from an internal restriction site. The DNA sequence contained an open reading frame of 558 nucleotides that would encode a protein of 21,268 daltons. Synthesis of such a protein was observed only when the fragment was cloned in front of the TAC promoter. Deletions entering the large open reading frame from either end were Fin0-, while internal frame shift mutations retained high Fin0 activity. One such strain had a 13 bp internal deletion that would produce a protein of 63 amino acid residues of which 21 were basic. We were consequently unable to rigorously establish that the 558 base orf encoded a fin0 product. The strand opposite the large open reading frame contained several transcription termination signals, and it is possible that the active gene product is one or two small RNAs from this strand.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=340615Documentos Relacionados
- Nucleotide sequence of gene X of antibiotic resistance plasmid R100.
- Nucleotide sequence analysis of the complement resistance gene from plasmid R100.
- Plasmid co-integrates of prophage lambda and R factor R100.
- oriT sequence of the antibiotic resistance plasmid R100.
- Integration host factor and conjugative transfer of the antibiotic resistance plasmid R100.