Filament Formation by Purified Physarum Myosin

AUTOR(ES)

Nachmias, Vivianne

RESUMO

Physarum myosin can be separated from actomyosin by ultracentrifugation, and purified by gel filtration. Unlike actomyosin, myosin is soluble in 0.05 M KCl in the pH range of 6-7. However, in the absence of actin, the slime mold myosin can be precipitated in 0.05 M KCl by the addition of millimolar concentrations of CaCl2. The precipitates consist of aggregated, short bipolar filaments. Magnesium has a similar effect, but results in the precipitation of more loosely packed aggregates.

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