Filamentous Bacterial Viruses VII. Inhibition of fd Deoxyribonucleic Acid Synthesis After a Temperature Jump into Protein-Synthesis Inhibitors

AUTOR(ES)

Tseng, B. Y.

RESUMO

Synthesis of fd deoxyribonucleic acid (DNA) was stopped by transferring infected bacteria from 32 C into chloramphenicol or serine hydroxamate at 42 C, but not by addition of these antibiotics at 32 C, and not by a temperature change in the absence of antibiotics. The inhibition of fd DNA synthesis by serine hydroxamate at 42 C was reversed by excess serine. The ability to synthesize fd DNA at 42 C in chloramphenicol was rescued by delaying the addition of chloramphenicol for a few minutes after the transfer from 32 to 42 C. The colony-forming ability of abortively infected bacteria was also rescued from “killing” by delaying the addition of chloramphenicol after a transfer from 32 to 42 C.

Documentos Relacionados

• Filamentous Bacterial Viruses V. Asymmetric Replication of fd Duplex Deoxyribonucleic Acid
• Filamentous Bacterial Viruses VI. Role of fd Gene 2 in Deoxyribonucleic Acid Replication
• Induced K+ Efflux from Cultured Rose Cells 1: Effects of Protein-Synthesis Inhibitors
• Memory Consolidation for the Discrimination of Frequency-Modulated Tones in Mongolian Gerbils Is Sensitive to Protein-Synthesis Inhibitors Applied to the Auditory Cortex
• Adenovirus-Associated Virus Multiplication VII. Helper Requirement for Viral Deoxyribonucleic Acid and Ribonucleic Acid Synthesis