Filamentous phage as vector-mediated antibody delivery to the brain
AUTOR(ES)
Frenkel, Dan
FONTE
National Academy of Sciences
RESUMO
Early diagnosis of Alzheimer's disease is prevented by lack of means to visualize and target β amyloid plaques in the brains of affected people. There are many methods of detecting amyloid plaques by staining postmortem brain tissue, but none are available for monitoring in living patients. We propose anti-β amyloid antibodies as a highly specific probe to monitor amyloid plaque formation in living patients. Intranasal administration of filamentous phage as delivery vector of anti-β amyloid antibody fragment into Alzheimer's APP transgenic mice enables in vivo targeting of β amyloid plaques. The plaques were covisualized both by thioflavin-S and fluorescent-labeled antiphage antibodies in the olfactory bulb and the hippocampus region. The genetically engineered filamentous bacteriophage proved to be an efficient and nontoxic viral delivery vector to the brain, offering an obvious advantage over other mammalian vectors. The ability to image Aβ deposits in vivo would arguably provide the most useful diagnostic and monitoring test for early diagnosis of Alzheimer's disease.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=122830Documentos Relacionados
- Pharmacologic effects in vivo in brain by vector-mediated peptide drug delivery.
- Vector-mediated delivery of a polyamide ("peptide") nucleic acid analogue through the blood-brain barrier in vivo.
- Adeno-associated virus vector-mediated IL-10 gene delivery prevents type 1 diabetes in NOD mice
- Antigen presentation in retroviral vector-mediated gene transfer in vivo
- High frequency vector-mediated transformation and gene replacement in Tetrahymena.