Fine-structure analysis of the DNA sequence requirements for autonomous replication of Saccharomyces cerevisiae plasmids.
AUTOR(ES)
Bouton, A H
RESUMO
An autonomously replicating segment, ARS, is located 293 base pairs downstream from the histone H4 gene at the copy-I H3-H4 locus. The sequences needed for autonomous replication were defined by deletion analysis to include an ARS consensus sequence and an additional 3'-flanking region. External deletions into the 3'-flanking yeast sequences resulted in a loss of replication function. However, disruptions of the required 3'-flanking domain by either 10-base-pair linker-scanning substitutions or larger internal deletions did not impair autonomous replication. Thus, replication is dependent upon a flanking chromosome domain, but not an exact DNA sequence. The extent of the yeast sequences required in the 3'-flanking domain is variable depending on the nature of neighboring plasmid vector sequences. That is, there are certain vector sequences that prohibit replication when they are placed too close to the ARS consensus. These results suggest that the functional 3'-flanking domain of the H4 ARS is a specific DNA or chromatin structure or both.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=367788Documentos Relacionados
- Fine-Structure Analysis of Intercellular and Intracellular Mitochondrial Diversity in Saccharomyces cerevisiae
- The CAN1 Locus of SACCHAROMYCES CEREVISIAE: Fine-Structure Analysis and Forward Mutation Rates
- UNIQUENESS OF THE FINE-STRUCTURE CONSTANT
- DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12.
- Fine-structure analysis of the P1 plasmid partition site.