Fine-Structure Mapping of Meiosis-Specific Double-Strand DNA Breaks at a Recombination Hotspot Associated with an Insertion of Telomeric Sequences Upstream of the His4 Locus in Yeast

AUTOR(ES)

Xu, F.

RESUMO

Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a recombination hotspot created by insertion of telomeric sequences into the region upstream of HIS4. We found that the breaks have no obvious sequence specificity and localize to a region of ~50 bp adjacent to the telomeric insertion. By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we conclude that most of the broken DNA molecules have blunt ends with 3'-hydroxyl groups.

Documentos Relacionados

• Meiosis-specific double-strand DNA breaks at the HIS4 recombination hot spot in the yeast Saccharomyces cerevisiae: control in cis and trans.
• Covalent protein-DNA complexes at the 5' strand termini of meiosis-specific double-strand breaks in yeast.
• Long Palindromic Sequences Induce Double-Strand Breaks during Meiosis in Yeast
• Sequence non-specific double-strand breaks and interhomolog interactions prior to double-strand break formation at a meiotic recombination hot spot in yeast.
• Genetic Evidence That the Meiotic Recombination Hotspot at the His4 Locus of Saccharomyces Cerevisiae Does Not Represent a Site for a Symmetrically Processed Double-Strand Break