Fission yeast retrotransposon Tf1 integration is targeted to 5′ ends of open reading frames

AUTOR(ES)

Behrens, Ralf

FONTE

Oxford University Press

RESUMO

Target site selection of transposable elements is usually not random but involves some specificity for a DNA sequence or a DNA binding host factor. We have investigated the target site selection of the long terminal repeat-containing retrotransposon Tf1 from the fission yeast Schizosaccharomyces pombe. By monitoring induced transposition events we found that Tf1 integration sites were distributed throughout the genome. Mapping these insertions revealed that Tf1 did not integrate into open reading frames, but occurred preferentially in longer intergenic regions with integration biased towards a region 100–420 bp upstream of the translation start site. Northern blot analysis showed that transcription of genes adjacent to Tf1 insertions was not significantly changed.

Documentos Relacionados

• Demonstration of retrotransposition of the Tf1 element in fission yeast.
• Novel gene expression mechanism in a fission yeast retroelement: Tf1 proteins are derived from a single primary translation product.
• Novel gene expression mechanism in a fission yeast retroelement: Tf1 proteins are derived from a single primary translation product
• Nup124p Is a Nuclear Pore Factor of Schizosaccharomyces pombe That Is Important for Nuclear Import and Activity of Retrotransposon Tf1
• The retrotransposon Tf1 assembles virus-like particles that contain excess Gag relative to integrase because of a regulated degradation process.