Flavin 1, N6-Ethenoadenine Dinucleotide: Dynamic and Static Quenching of Fluorescence

AUTOR(ES)

Barrio, Jorge R.

RESUMO

Flavin 1,N6-ethenoadenine dinucleotide (εFAD) was prepared by the action of chloroacetaldehyde on flavin adenine dinucleotide. This compound, which has two potential fluorophores, ε-adenine and isoalloxazine, shows extremely efficient energy transfer from the former to the latter. The fluorescences of both moieties are greatly diminished in the intact molecule. Determination of the fluorescence yields and lifetimes leads to the conclusion that at 20° in neutral aqueous solution εFAD exists mainly (90%) as an internally complexed or stacked form.

Documentos Relacionados

• Dynamic and static quenching of 1,N6-ethenoadenine fluorescence in nicotinamide 1,N6-ethenoadenine dinucleotide and in 1,N6-etheno-9-(3-(indol-3-yl) propyl) adenine.
• Both purified human 1,N6-ethenoadenine-binding protein and purified human 3-methyladenine-DNA glycosylase act on 1,N6-ethenoadenine and 3-methyladenine.
• Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique
• Flavin adenine dinucleotide as a chromophore of the Xenopus (6-4)photolyase.
• Targeted deletion of alkylpurine-DNA-N-glycosylase in mice eliminates repair of 1,N6-ethenoadenine and hypoxanthine but not of 3,N4-ethenocytosine or 8-oxoguanine