FlgM is a primary regulator of sigmaD activity, and its absence restores motility to a sinR mutant.

AUTOR(ES)

Fredrick, K

RESUMO

We have used mini-Tn1O mutagenesis to identify negative regulators of sigmaD activity. Nine independent insertions were mapped to five genes: flgM, flgK, fliD, fliS, and fliT, suggesting that FlgM export is regulated similarly in Bacillus subtilis and Salmonella typhimurium. We show that a deletion of flgM can restore sigmaD activity to a sinR null mutant of B. subtilis, although fla/che operon expression is affected by neither SinR nor FlgM.

Documentos Relacionados

• Role of FlgM in sigma D-dependent gene expression in Bacillus subtilis.
• The attenuated phenotype of a Salmonella typhimurium flgM mutant is related to expression of FliC flagellin.
• The alternative sigma factor sigma28 of Legionella pneumophila restores flagellation and motility to an Escherichia coli fliA mutant.
• FlgM gains structure in living cells
• The Bacillus subtilis regulator SinR inhibits spoIIG promoter transcription in vitro without displacing RNA polymerase.