Fluid accumulation in infant mice caused by Vibrio hollisae and its extracellular enterotoxin.
AUTOR(ES)
Kothary, M H
RESUMO
Vibrio hollisae, a halophilic bacterium isolated from patients with diarrhea, was examined for virulence factor production. Intragastric administration of 2 X 10(7) CFU per mouse elicited fluid accumulation which peaked at ca. 6 h postchallenge in infant mice. An enterotoxin which elongated Chinese hamster ovary (CHO) cells was detected in extracts of infected-mouse intestines and in culture fluids from various growth media. The yield of the enterotoxin was maximal beginning at the onset of the stationary phase of growth in heart infusion broth supplemented with 0.5% NaCl. A concentrated preparation obtained by ammonium sulfate precipitation of culture supernatant fluids induced intestinal fluid accumulation which peaked at 2 h postchallenge in infant mice. The abilities of the enterotoxin preparation to elongate CHO cells and to elicit fluid accumulation in infant mice were inseparable by gel filtration, isoelectric focusing, and hydrophobic interaction chromatography. The enterotoxin has a molecular weight of ca. 33,000 by gel filtration and an isoelectric point of ca. 4 and is sensitive to heat.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=260385Documentos Relacionados
- Bacteremia caused by Vibrio hollisae.
- Intestinal fluid accumulation induced by oral challenge with Vibrio cholerae or cholera toxin in infant mice.
- Fluid accumulation in mouse ligated intestine inoculated with Clostridium perfringens enterotoxin.
- Failure of chlorpromazine to inhibit fluid accumulation caused by Escherichia coli heat-stable enterotoxin in suckling mice.
- Swiss and inbred mice in the infant mouse test for the assay of Escherichia coli thermostable enterotoxin.