Form of Vitamin B12 and Its Role in a Methanol-Utilizing Bacterium Protaminobacter ruber
AUTOR(ES)
Sato, Kazuyoshi
RESUMO
A methanol-utilizing bacterium, Protaminobacter ruber, produced a large amount of vitamin B12. The compounds were isolated from the cells and identified as methylcobalamin (methyl-B12) and adenosylcobalamin (adenosyl-B12) by various tests. The variation in the form of B12 during cultivation was examined by bioautography with cellulose acetate membrane electrophoresis. Methyl-B12 and adenosyl-B12 were the two main B12 compounds produced in the various phases of bacterial growth. The ratio of the amount of methyl-B12 to total B12 compounds was higher during the earlier phases of growth. After the logarithmic phase, adenosyl-B12 was the predominant form. The existence of N5-methyltetrahydrofolate:homocysteine transmethylase and methyl-B12:homocysteine transmethylase was demonstrated in cell-free extracts of Protaminobacter ruber. Methyl-B12 in P. ruber seems to function mainly in the B12-dependent methionine synthetase system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=170718Documentos Relacionados
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