Formation of a Functional Electron Transport System During Growth of Penicillin-Induced Spheroplasts of Haemophilus parainfluenzae
AUTOR(ES)
Wright, Elizabeth A.
RESUMO
Wright, Elizabeth A. (University of Kentucky College of Medicine, Lexington), and David C. White. Formation of a functional electron transport system during growth of penicillin-induced spheroplasts of Haemophilus parainfluenzae. J. Bacteriol. 91:1356–1362. 1966.—Penicillin in a lactose medium can be used to cause the formation of spheroplasts in Haemophilus parainfluenzae. The resulting spheroplasts grew under conditions which produced rapid formation of the electron transport system in the normal bacteria. The following elements that are incorporated into a functionally active electron transport system were formed in spheroplasts: formate and l-lactate dehydrogenases, 2-demethyl vitamin K2, cytochromes b1 and c1, and the cytochrome oxidases. The catabolic enzymes aldolase, glyceraldehyde-3-phosphate dehydrogenase, and malic dehydrogenase showed slight increases in activity. These experiments indicated that spheroplasts can form a fully functional electron transport system essentially similar to that formed during normal growth. The various components of the electron transport system were formed at different rates in the growing spheroplasts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=316035Documentos Relacionados
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