Formation of Adenosine 5′-Phosphorofluoridate and the Assay of Adenyl Cyclase in Barley Seeds 1

AUTOR(ES)

Alvarez, Robert

RESUMO

Barley seed (Hordeum vulgare L.) homogenates contain an apparent enzymatic activity which catalyzes the synthesis of adenosine 5′-phosphorofluoridate from magnesium-adenosine 5′-triphosphate and sodium fluoride. Formation of this compound may interfere with some adenyl cyclase assays which use fluoride as a component of the incubation medium. Neither adenyl cyclase activity nor endogenous adenosine 3′: 5′-monophosphate was detected in barley seed homogenates or extracts.

Documentos Relacionados

• STIMULATION OF ANTERIOR PITUITARY ADENYL CYCLASE ACTIVITY AND ADENOSINE 3′:5′-CYCLIC PHOSPHATE BY HYPOTHALAMIC EXTRACT AND PROSTAGLANDIN E1*
• Effects of vasopressin and prostaglandin E1 on the adenyl cyclase—cyclic 3′,5′-adenosine monophosphate system of the renal medulla of the rat
• Properties of adenyl cyclase and cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli.
• Effects of Cholera Enterotoxin on Adenosine 3′,5′-Monophosphate and Neutrophil Function. COMPARISON WITH OTHER COMPOUNDS WHICH STIMULATE LEUKOCYTE ADENYL CYCLASE
• Myocardial adenyl cyclase: activation by thyroid hormones and evidence for two adenyl cyclase systems