Formation of novel hairpin structures by telomeric C-strand oligonucleotides.

AUTOR(ES)

Ahmed, S

RESUMO

Telomeres are specialized structures at the ends of chromosomes that are required for long term chromosome stability and replication of the chromosomal terminus. Telomeric DNA consists of simple repetitive sequences with one strand G-rich relative to the other, C-rich, strand. Evolutionary conservation of this feature of telomeric repeat sequences suggests that they have specific structural characteristics involved in telomere function. Absorbance thermal denaturation, chemical modification and non-denaturing gel electrophoretic analyses showed that telomeric C-strand oligonucleotides form stable non-Watson-Crick hairpin structures containing C.C+ base pairs. Formation of such hairpins may facilitate previously reported G-strand exclusive interactions.

Documentos Relacionados

• Single strand targeted triplex-formation. Destabilization of guanine quadruplex structures by foldback triplex-forming oligonucleotides.
• Inhibition of dengue virus by novel, modified antisense oligonucleotides.
• Hairpin and parallel quartet structures for telomeric sequences.
• Formation of a parallel-stranded DNA homoduplex by d(GGA) repeat oligonucleotides.
• Sequence-specific DNA double-strand breaks induced by triplex forming 125I labeled oligonucleotides.