Formation of recombinants between snowshoe hare and La Crosse bunyaviruses.
AUTOR(ES)
Gentsch, J
RESUMO
Wild-type recombinants were obtained at high frequency from coinfections of BHK cells involving temperature-sensitive, conditional-lethal mutants of snowshoe hare (SSH) and La Crosse (LAC) bunyaviruses. Analyses of two of the recombinants indicated that they have the genome compositions SSH/LAC/SSH and SSH/LAC/LAC for their respective L, M, and S virion RNA species. This evidence, together with that for the genetic stability of the recombinants, indicates that they were derived by segment reassortment of the competent genome pieces of the parental viruses. The SSH/LAC/SSH recombinant appears, from polypeptide analysis, to have the SSH type of nucleocapsid protein (N), whereas the SSH/LAC/LAC recombinant has the LAC nucleocapsid protein, suggesting that the viral S RNA codes for the N protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=516011Documentos Relacionados
- Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses.
- Recombination between snowhoe hare and La Crosse bunyaviruses.
- Comparison of the sequences and coding of La Crosse and snowshoe hare bunyavirus S RNA species.
- Typing of LaCrosse, snowshoe hare, and Tahyna viruses by analyses of single-strand conformation polymorphisms of the small RNA segments.
- Population regulation in snowshoe hare and Canadian lynx: Asymmetric food web configurations between hare and lynx