Function of Individual 30S Subunit Proteins of Escherichia coli. Effect of Specific Immunoglobulin Fragments (Fab) on Activities of Ribosomal Decoding Sites

AUTOR(ES)

Lelong, J. C.

RESUMO

Specific anti-30S protein immunoglobulin G fragments (Fab) were used to determine the contribution of each of the 30S ribosomal proteins to: (1) polyphenylalanine synthesis, (2) initiation factor-dependent binding of fMet-tRNA, (3) T-factor-dependent binding of phenylalanyl-tRNA, and (4) fixation of radioactive dihydrostreptomycin. Twenty of the 21 possible antibodies (antibody against S17 excepted) were used. In conditions where all the 30S proteins were accessible to Fabs, all of these monovalent antibodies strongly inhibited polyphenylalanine synthesis in vitro. Antibodies against S4, S6, S7, S12, S15, and S16, however, showed a weaker effect.

Documentos Relacionados

• Localization of the decoding region on the 30S Escherichia coli ribosomal subunit by affinity immunoelectron microscopy.
• Interactions between 30s ribosomal proteins and 50s subunits of Escherichia coli.
• The conformation of 16S RNA in the 30S ribosomal subunit from Escherichia coli.
• Function and properties of the "native" 30S and 50S ribosomal subunits of Escherichia coli.
• Effect of Colicin E3 upon the 30S Ribosomal Subunit of Escherichia coli