Function of simian virus 40 gene A in transforming infection.
AUTOR(ES)
Tegtmeyer, P
RESUMO
In productive infection by simian virus 40, the A gene is known to regulate the initiation of viral DNA replication and to control the synthesis of late viral RNA. The function of the A gene in transforming infection was investigated by the infection of a variety of cell species with six independently isolated temperature-sensitive mutants belonging to the A complementation group. The A mutants failed to initiate the stable transformation of cells during continuous infection at the restrictive temperature. After the establishment of transformation at the permissive temperature and a subsequent shift to the restrictive temperature to block the A function, however, two distinct virus-cell interactions were identified. In one case, the increased colony-forming capacity of transformed cells remained stable after the temperature shift. In the other case, the temperature shift decreased the capacity of transformed cells to form colonies to the level of untransformed control cells. The outcome of the virus-cell interaction depended both on the nature of the A mutation in a given cell species and on the species of the cell transformed by a given mutant. These findings suggest that the transformation process may require two distinct events, each related to A gene expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354497Documentos Relacionados
- Adenovirus type 12 gene 401 function in transforming infection.
- Intracellular localization of viral polypeptides during simian virus 40 infection.
- Subcellular distribution of viral structural proteins during simian virus 40 infection.
- Simian virus 40 large T-antigen function is required for induction of tetraploid DNA content during lytic infection.
- Role of simian virus 40 gene A function in maintenance of transformation.