Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca2+ Transients in Ciliated Tracheal Epithelial Cells
AUTOR(ES)
Groathouse, Nathan A.
FONTE
American Society for Microbiology
RESUMO
To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=308951Documentos Relacionados
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