Functional characterization of the interferon regulatory element in the enhancer 1 region of the hepatitis B virus genome
AUTOR(ES)
Alcantara, Flavio F.
FONTE
Oxford University Press
RESUMO
An interferon-stimulated response element (ISRE)/interferon regulatory element (IRE) spanning nucleotide coordinates 1091–1100 is present in the enhancer 1/X gene promoter region of the hepatitis B virus (HBV) genome. In the context of a minimal promoter element, the enhancer 1/X gene promoter ISRE/IRE was shown to be a functional regulatory site capable of mediating interferon α- (IFNα) and interferon-stimulated gene factor 3 (ISGF3)-specific transcriptional activation in transient transfection analysis. The enhancer 1/X gene promoter ISRE/IRE was also shown to mediate interferon regulatory factor (IRF) 1 and IRF7 activation of transcription from a minimal promoter construct. In contrast, IFNα and the IRFs had minimal effect on HBV transcription and replication in the context of the viral genome in cell culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=113846Documentos Relacionados
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