Functional epithelial cell line cloned from rat parathyroid glands.
AUTOR(ES)
Sakaguchi, K
RESUMO
Primary cultures of rat parathyroid cells were developed in medium containing 5% calf serum, 1% Nutridoma-SP (a serum-free medium supplement from Boehringer Mannheim), and 0.7 mM calcium. The PT-r strain was purified by successive colony isolations and maintained differentiated characteristics (secretion of bioactive and radioimmunoactive parathyroid hormone into the culture medium, sensitivity to calcium regulation, and modulation by secretin) for 7 months in continuous culture. These cultured cells are epithelioid, display diploid chromosome numbers, and do not show a transformed phenotype. There has been no decrease in the rate of cell division or decline in parathyroid hormone secretion since the cell line was established. This clonal cell line provides an important system for further studies on the biology of the parathyroid cell.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=304850Documentos Relacionados
- Letter: Mast cells in parathyroid glands.
- Epithelial-myoepithelial carcinoma of salivary glands.
- Calcium-dependent chloride currents in isolated cells from rat lacrimal glands.
- Hyperparathyroidism associated with the enlargement of two or three parathyroid glands.
- Electric current flow in a two-cell preparation from Chironomus salivary glands.