Functional role of the zipper motif region of human immunodeficiency virus type 1 transmembrane protein gp41.
AUTOR(ES)
Chen, S S
RESUMO
To study the functional role of the zipper motif region, located in the N-terminal region of the envelope transmembrane protein of human immunodeficiency virus type 1, a series of vaccinia virus-expressed mutant proteins containing a proline substitution in this region were characterized. All of the mutant proteins showed partial or no inhibition in gp160 cleavage, demonstrated impaired ability of gp120 to associate with gp41, and were unable to mediate syncytium formation with CD4+ cells. Moreover, mutants 580 and 587 secreted excessive gp120 into the medium compared with the wild type. Mutations in this region affected the conformation of the local or proximal sequence but did not alter the conformation conferred by a distal site. These studies reveal the crucial role of the C-terminal segment of the zipper motif region in envelope heterodimeric association and suggest that this sequence forms a gp120 contact site.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=236667Documentos Relacionados
- Biophysical characterization of recombinant proteins expressing the leucine zipper-like domain of the human immunodeficiency virus type 1 transmembrane protein gp41.
- Membrane permeabilization by different regions of the human immunodeficiency virus type 1 transmembrane glycoprotein gp41.
- Functional role of the glycan cluster of the human immunodeficiency virus type 1 transmembrane glycoprotein (gp41) ectodomain.
- Oligomeric structure of gp41, the transmembrane protein of human immunodeficiency virus type 1.
- Characterization of the fusion domain of the human immunodeficiency virus type 1 envelope glycoprotein gp41.