Functionally Heterogeneous CD8+ T-Cell Memory Is Induced by Sendai Virus Infection of Mice
AUTOR(ES)
Usherwood, Edward J.
FONTE
American Society for Microbiology
RESUMO
It has recently been established that memory CD8+ T cells induced by viral infection are maintained at unexpectedly high frequencies in the spleen. While it has been established that these memory cells are phenotypically heterogeneous, relatively little is known about the functional status of these cells. Here we investigated the proliferative potential of CD8+ memory T cells induced by Sendai virus infection. High frequencies of CD8+ T cells specific for both dominant and subdominant Sendai virus epitopes persisted for many weeks after primary infection, and these cells were heterogeneous with respect to CD62L expression (approximately 20% CD62Lhi and 80% CD62Llo). Reactivation of these cells with the antigenic peptide in vitro induced strong proliferation of antigen-specific CD8+ T cells. However, approximately 20% of the cells failed to proliferate in vitro in response to a cognate peptide but nevertheless differentiated into effector cells and acquired full cytotoxic potential. These cells also expressed high levels of CD62L (in marked contrast to the CD62Llo status of the proliferating cells in the culture). Direct isolation of CD62Lhi and CD62Llo CD8+ T cells from memory mice confirmed the correlation of this marker with proliferative potential. Taken together, these data demonstrate that Sendai virus infection induces high frequencies of memory CD8+ T cells that are highly heterogeneous in terms of both their phenotype and their proliferative potential.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=104253Documentos Relacionados
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