Fungicidal activation of murine macrophages by recombinant gamma interferon.
AUTOR(ES)
Beaman, L
RESUMO
Alveolar macrophages from BALB/c mice readily phagocytized endospores (2 to 5 micron) and arthroconidia of Coccidioides immitis in vitro. Within 24 to 30 h at 37 degrees C, the phagocytized endospores started developing into spherules, and the arthroconidia formed germ tubes and hyphae. However, these processes did not occur if the macrophages were incubated with murine recombinant gamma interferon (rIFN-gamma) during infection with C. immitis. Treatment with rIFN-gamma activated the fungicidal capabilities of the alveolar macrophages, as evidenced by the 50% reduction in the CFU which could be recovered from macrophages infected in the presence of gamma interferon compared with alveolar macrophages infected without gamma interferon (P less than 0.05). Similar results were seen with peritoneal macrophages incubated with rIFN-gamma and infected with C. immitis. As little as 10 U of rIFN-gamma per ml reduced by half the number of C. immitis CFU which could be recovered from the phagocytes 8 h after infection with arthroconidia, although interferon alone did not affect the viability of the fungi.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=260012Documentos Relacionados
- Mechanism for candidacidal activity in macrophages activated by recombinant gamma interferon.
- Mycobacterium leprae-burdened macrophages are refractory to activation by gamma interferon.
- Inhibition of the intracellular growth of Histoplasma capsulatum by recombinant murine gamma interferon.
- In vivo immune responses to Candida albicans modified by treatment with recombinant murine gamma interferon.
- Enhancement of growth of Mycobacterium lepraemurium in macrophages by gamma interferon.
