Generation of defective interfering particles of Semliki Forest virus in a clone of Aedes albopictus (mosquito) cells.
AUTOR(ES)
Logan, K B
RESUMO
Serial undiluted passage of Semliki Forest virus in a clone of Aedes albopictus cells resulted in a marked decrease in infectious virus yields due to the generation and accumulation of defective interfering particles. Virus from the third passage had a high particle/infectivity ratio and interfered specifically with homologous but not heterologous standard virus replication. Two RNA species of molecular weights 0.78 X 10(6) and 0.61 X 10(6) were the major RNA components of purified passage 4 virus. These RNA species were also the predominant virus RNA species detected in cells infected with passage 3 virus. Synthesis of standard virus RNA and virus-specified protein was much reduced in passage 3 virus-infected cells. Interference with standard virus replication and the synthesis of large amounts of defective interfering RNA were also observed in chicken embryo cells infected with passage 3 virus from mosquito cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=353295Documentos Relacionados
- Morphogenesis of Sindbis virus in three subclones of Aedes albopictus (mosquito) cells.
- Exclusion of superinfecting homologous virus by Sindbis virus-infected Aedes albopictus (mosquito) cells.
- Semliki Forest virus multiplication in clones of Aedes albopictus cells.
- Effect of actinomycin D and cycloheximide on replication of Sindbis virus in Aedes albopictus (mosquito) cells.
- Characteristics of Sindbis virus temperature-sensitive mutants in cultured BHK-21 and Aedes albopictus (Mosquito) cells.