Genes encoding enzymes of the lignin biosynthesis pathway in Eucalyptus
AUTOR(ES)
Harakava, Ricardo
FONTE
Genetics and Molecular Biology
DATA DE PUBLICAÇÃO
2005
RESUMO
Eucalyptus ESTs libraries were screened for genes involved in lignin biosynthesis. This search was performed under the perspective of recent revisions on the monolignols biosynthetic pathway. Eucalyptus orthologues of all genes of the phenylpropanoid pathway leading to lignin biosynthesis reported in other plant species were identified. A library made with mRNAs extracted from wood was enriched for genes involved in lignin biosynthesis and allowed to infer the isoforms of each gene family that play a major role in wood lignin formation. Analysis of the wood library suggests that, besides the enzymes of the phenylpropanoids pathway, chitinases, laccases, and dirigent proteins are also important for lignification. Colocalization of several enzymes on the endoplasmic reticulum membrane, as predicted by amino acid sequence analysis, supports the existence of metabolic channeling in the phenylpropanoid pathway. This study establishes a framework for future investigations on gene expression level, protein expression and enzymatic assays, sequence polymorphisms, and genetic engineering.
Documentos Relacionados
- Sugarcane expressed sequences tags (ESTs) encoding enzymes involved in lignin biosynthesis pathways
- An alternative methylation pathway in lignin biosynthesis in Zinnia.
- Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
- A cluster of four genes encoding enzymes for five steps in the folate biosynthetic pathway of Streptococcus pneumoniae.
- Clustered Pathway Genes in Aflatoxin Biosynthesis