Genetic Analysis of Mouse T Haplotypes Using Mutations Induced by Ethylnitrosourea Mutagenesis: The Order of T and Qk Is Inverted in T Mutants

AUTOR(ES)

Justice, M. J.

RESUMO

The t region of mouse chromosome 17 exhibits recombination suppression with wild-type chromatin. However, the region has resisted classical genetic dissection because of a lack of defined variants. Mutations induced by N-ethyl-N-nitrosourea (ENU) at the Brachyury (T), quaking (qk), and tufted (tf) loci of the mouse t(w5) haplotype have now allowed the analysis of crossovers between two complete t haplotypes. A classical breeding analysis of the complete t haplotypes, t(w5) and t(12), utilizing the newly induced markers, reveals two inversions in t chromatin: one involving T and qk, and one involving tf and the H-2 complex. Moreover, the recombination frequency between the loci of T and qk is reduced compared to the frequency reported in normal chromatin. These two inversions are a sufficient explanation for the recombination inhibition with normal chromatin exhibited by t haplotypes isolated from the wild. Furthermore, the reduced recombination frequency between T and qk may indicate that the proximal gene rearrangement is not a simple inversion.

Documentos Relacionados

• Electrophoretically detected germinal mutations induced in the mouse by ethylnitrosourea.
• Deletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutants.
• Genetic and Molecular Analysis of the Proximal Region of the Mouse T-Complex Using New Molecular Probes and Partial T-Haplotypes
• sar: a genetic mouse model for human sarcosinemia generated by ethylnitrosourea mutagenesis.
• Hph-1: A Mouse Mutant with Hereditary Hyperphenylalaninemia Induced by Ethylnitrosourea Mutagenesis