Genetic Analysis of Peptidoglycan Biosynthesis in Mycobacteria: Characterization of a ddlA Mutant of Mycobacterium smegmatis

AUTOR(ES)

Belanger, Aimee E.

FONTE

American Society for Microbiology

RESUMO

A temperature-sensitive mutant of Mycobacterium smegmatis was characterized that contains a mutation in ddlA, the gene encoding d-alanine:d-alanine ligase. Enzymatic assays using recombinant proteins and d-cycloserine susceptibility indicate that the A365V mutation in the SMEG35 DdlA protein causes a reduction in enzymatic activity in vitro and in vivo.

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