Genetic and Molecular Analysis of the Proximal Region of the Mouse T-Complex Using New Molecular Probes and Partial T-Haplotypes

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The t-complex is located on the proximal third of chromosome 17 in the house mouse. Naturally occuring variant forms of the t-complex, known as complete t-haplotypes, are found in wild mouse populations. The t-haplotypes contain at least four nonoverlapping inversions that suppress recombination with the wild-type chromosome, and lock into strong linkage disequilibrium loci affecting normal transmission of the chromosome, male gametogenesis and embryonic development. Partial t-haplotypes derived through rare recombination between t-haplotypes and wild-type homologs have been critical in the analysis of these properties. Utilizing two new DNA probes, Au3 and Au9, and several previously described probes, we have analyzed the genetic structure of several partial t-haplotypes that have arisen in our laboratory, as well as several wild-type chromosomes deleted for loci in this region. With this approach we have been able to further our understanding of the structural and dynamic characteristics of the proximal region of the t-complex. Specifically, we have localized the D17Tu1 locus as most proximal known in t-haplotypes, achieved a better structural analysis of the partial t-haplotype t(6), and defined the structure and lethal gene content of partial t-haplotypes derived from the lethal t(w73) haplotype.

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