Genetic Diversity of Gallibacterium anatis Isolates from Different Chicken Flocks

AUTOR(ES)

Bojesen, Anders Miki

FONTE

American Society for Microbiology

RESUMO

Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were characterized by more than 94% genetic similarity, indicating that only a single clone was apparent in the flock. The layer parent flock isolates were grouped into two subclusters, each with similarity above 90%. One subcluster contained only tracheal isolates, while the other primarily included cloacal isolates. In conclusion, we show that AFLP analysis enables fingerprinting of G. anatis, which seems to have a clonal population structure within natural populations. There was further evidence of clonal lineages, which may have adapted to different sites within the same animal.

Documentos Relacionados

• Diagnosis of Gallibacterium Anatis in Layers: First Report in Turkey
• Genetic diversity of Plasmodium vivax isolates from Azerbaijan
• Molecular Phylogenies of Blastocystis Isolates from Different Hosts: Implications for Genetic Diversity, Identification of Species, and Zoonosis
• Genetic Diversity of Isolates of Glomus mosseae from Different Geographic Areas Detected by Vegetative Compatibility Testing and Biochemical and Molecular Analysis
• Phenotypic and Genetic Diversity of Invasive Pneumococcal Isolates Recovered from French Children