Genetic manipulation of major P-fimbrial subunits and consequences for formation of fimbriae.

AUTOR(ES)

Van Die, I

RESUMO

The influence of genetic manipulation of the structural genes coding for major P-fimbrial subunits on the formation of fimbriae in Escherichia coli was studied. Deletion of two regions that code for hypervariable parts of the P fimbrillin resulted in strong reduction or total absence of fimbria production. Replacement of deleted amino acids by other amino acid residues restored the formation of fimbriae. The hypervariable regions may be important for biogenesis of fimbriae by imposing correct spacing between conserved regions of the protein. The potential for substituting amino acids in the P-fimbrial subunit opens interesting possibilities for use of fimbriae as carriers of foreign antigenic determinants. An antigenic determinant of foot-and-mouth disease virus (FMDV) was incorporated in the F11 fimbrial subunit. Hybrid fimbriae, recognized by an FMDV-specific neutralizing monoclonal antibody directed against FMDV, were formed.

Documentos Relacionados

• Prevalence of Known P-Fimbrial G Alleles in Escherichia coli and Identification of a New Adhesin Class
• Identification of minor fimbrial subunits involved in biosynthesis of K88 fimbriae.
• Induction of protective immunity after escherichia coli bladder infection in primates. Dependence of the globoside-specific P-fimbrial tip adhesin and its cognate receptor.
• Identification of major and minor chaperone proteins involved in the export of 987P fimbriae.
• Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae.