Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12.
AUTOR(ES)
White, B J
RESUMO
The genes xthA, pncA, and pabB were ordered relative to others by two- and three-factor transductional crosses with bacteriophage P1. The genes studied span 2 min (2%) of the genetic map of Escherichia coli K-12 in the clockwise sequence pheS-pfkB-xthA-pncA-gap-pabB-fadD. Eleven independently derived xth mutations were examined; all were known to affect exonuclease III and its associated endonuclease II activity, and all were mapped in the xthA region. pncA mutations were found to confer resistance to 6-aminonicotinamide, whereas some pheS mutations are known to specify resistance to p-fluorphenylalanine. xth mutations were readily transferred into other strains by selecting for these co-transducible drug resistance markers.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=233128Documentos Relacionados
- Nucleotide sequence of the xth gene of Escherichia coli K-12.
- Genetic mapping of nth, a gene affecting endonuclease III (thymine glycol-DNA glycosylase) in Escherichia coli K-12.
- Preliminary Mapping of Mutations Affecting Exonuclease III in Escherichia coli K-12
- Mapping of the gene for cytidine deaminase (cdd) in Escherichia coli K-12.
- Nucleotide sequence of the structural gene for tryptophanase of Escherichia coli K-12.
