Genetically Induced Subcellular Mislocation of Neurospora Mitochondrial Malate Dehydrogenase
AUTOR(ES)
Munkres, K. D.
RESUMO
Among 60 ultraviolet-induced missense mutations of the structural genes that code for mitochondrial malate dehydrogenase (M-MDH, EC 1.1.1.37) of Neurospora crassa, two enzyme phenotypes are observed. In a previously described class (C-mutants), M-MDH is malfunctional because of an abnormal conformation induced by association with mitochondria. We describe here a second class (K-mutants) in which the enzyme is malfunctional because of an altered subcellular location. Thus, although both classes cause lesions in the assimilation of exogenous malate, the nature of the lesions differs. In C-mutants, the enzyme misfunctions because of low affinity for malate but remains mitochondrial-bound as in wild-type. Conversely, K-mutant M-MDH is dispersed throughout the cytoplasm.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=283197Documentos Relacionados
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