Genome of avian myelocytomatosis virus MC29: analysis by heteroduplex mapping.

AUTOR(ES)

Hu, S S

RESUMO

The virion RNA of avian myelocytoma virus MC29 was hybridized to full genome length DNA of the Prague strain of Rous sarcoma virus and analyzed by heteroduplex mapping in the electron microscopy. The results show that MC29 specific sequences for which there are no homologous counterparts in the Rous sarcoma virus genome make up a contiguous stretch of RNA about 1.8 kilobases long. These sequences are located approximately in the middle of the genome, replacing the 3' half of the gag gene, the entire pol gene, and the 5' portion of the env gene, which are absent from MC29. This MC29 specific genetic substitution may contain information for the leukemogenic transformation of the host cell.

Documentos Relacionados

• Avian reticuloendotheliosis virus: characterization of genome structure by heteroduplex mapping.
• Nucleotide sequence analysis of the proviral genome of avian myelocytomatosis virus (MC29).
• Transfection by DNAs of avian erythroblastosis virus and avian myelocytomatosis virus strain MC29.
• Molecular cloning of avian myelocytomatosis virus (MC29) transforming sequences.
• Characterization of the env gene in avian oncoviruses by heteroduplex mapping.