Genomic expansion of Marek's disease virus DNA is associated with serial in vitro passage.
AUTOR(ES)
Silva, R F
RESUMO
An EcoRI restriction endonuclease pattern of Md11 virus DNA, a very virulent strain of Marek's disease virus (MDV), was obtained by using total cellular DNA from infected cells. With the EcoRI restriction endonuclease pattern and a published BamHI map of MDV (Fukuchi et al., J. Virol. 51:102-109), we constructed a partial EcoRI map of a series of MDV clones (gift from H. J. Kung). The clones were used to identify a region of the Md11 genome which is altered as the oncogenic virus is passaged in vitro. This region was mapped into a 1.8-kilobase segment in the inverted-repeat sequences flanking the long unique region of the virus genome. The alteration appeared to result from multiple DNA insertions that produced an increase of 0.6 to 5.4 kilobases. Although the expansion of this region did not diminish the ability of MDV to replicate in vitro, it may be associated with the loss of Marek's disease oncogenicity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=254853Documentos Relacionados
- Amplification of a tandem direct repeat within inverted repeats of Marek's disease virus DNA during serial in vitro passage.
- In vitro cytotoxicity against Marek's disease lymphoblastoid cell lines after enzymatic removal of Marek's disease tumor-associated surface antigen.
- DNA Configuration in the Core of Marek's Disease Virus
- Growth hormone interacts with the Marek's disease virus SORF2 protein and is associated with disease resistance in chicken
- Preparation of Infectious Cell-Free Herpes-Type Virus Associated with Marek's Disease
