Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes.

AUTOR(ES)

Regnery, R L

RESUMO

DNA sequences from specific genes, amplified by the polymerase chain reaction technique, were used as substrata for nonisotopic restriction endonuclease fragment length polymorphism differentiation of rickettsial species and genotypes. The products amplified using a single pair of oligonucleotide primers (derived from a rickettsial citrate synthase gene sequence) and cleaved with restriction endonucleases were used to differentiate almost all recognized species of rickettsiae. A second set of primers was used for differentiation of all recognized species of closely related spotted fever group rickettsiae. The procedure circumvents many technical obstacles previously associated with identification of rickettsial species. Multiple amplified DNA digest patterns were used to estimate the intraspecies nucleotide sequence divergence for the genes coding for rickettsial citrate synthase and a large antigen-coding gene of the spotted fever group rickettsiae. The estimated relationships deduced from these genotypic data correlate reasonably well with established rickettsial taxonomic schemes.

Documentos Relacionados

• Structure and sequence divergence of two archaebacterial genes.
• Repetitive sequence involvement in the duplication and divergence of mouse lysozyme genes.
• Genotypic and antigenic identification of two new strains of spotted fever group rickettsiae isolated from China.
• Divergence and differential expression of soybean actin genes.
• Sequence divergence in two tandemly located pilin genes of Eikenella corrodens.