Germ Line Basis for Antibody Diversity: Immunoglobulin VH- and CH-gene Frequencies Measured by DNA·RNA Hybridization
AUTOR(ES)
Premkumar, E.
RESUMO
The reiteration frequency for mouse immunoglobulin VH-genes and CH-genes has been directly estimated by hybridization of purified MOPC 315 α-chain mRNA with a vast excess of mouse DNA. A biphasic Cot curve resulted. The low Cot transition (Cot1/2 about 1.5) was interpreted as hybridization to VH-genes and the high Cot transition (Cot1/2 about 103) as hybridization to CH-genes. These values correspond to about 5000 VH-genes and less than 8 CH-genes. This germ-line content of VH-genes is sufficient to account for antibody diversity given a comparable set of VL-genes. Minimal-gene models are invalidated and there is no need to invoke somatic generators of diversity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=387941Documentos Relacionados
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