Germination of Clostridium cylindrosporum Spores on Medium Containing Uric Acid
AUTOR(ES)
Smith, M.
RESUMO
Clostridium cylindrosporum spores germinated rapidly under reducing conditions when bicarbonate, uric acid, and calcium were present. Germination rates on 10 mM urate increased with increasing Ca2+ (maximum rate at 5 mM Ca2+ or greater). Germination rates on urate (limiting Ca2+) increased with increasing urate concentrations to 10 mM urate. At 10 mM Ca2+, germination rates reached a maximum at 1 mM urate and remained constant thereafter. Cations (Na+, K+, Li+, and Mg2+), purines, purine analogs, and EDTA inhibited germination at limiting calcium concentrations but not (except for 10 mM adenine) at 10 mM Ca2+. Methyl viologen or formate did not inhibit germination. Germination was not observed in solutions containing xanthine, hypoxanthine, caffeine, theophylline, 6,8-dihydroxypurine, adenine, allopurinol, formate, glycine, or acetate, even though some of the purines are growth substrates.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202875Documentos Relacionados
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