Glutamine Synthetase in Spinach Leaves 1: IMMUNOLOGICAL STUDIES AND IMMUNOCYTOCHEMICAL LOCALIZATION
AUTOR(ES)
Hirel, Bertrand
RESUMO
By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular localization of glutamine synthetase in spinach leaves was studied by indirect immunofluorescence microscopy on thin-sectioned spinach leaves. It has been demonstrated that the enzyme is specifically associated with the chloroplasts of parenchymatous cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426340Documentos Relacionados
- Immunocytolocalization of Glutamine Synthetase in Green Leaves and Cotyledons of Lycopersicon esculentum1
- Glutamine Synthetase in Rice: A COMPARATIVE STUDY OF THE ENZYMES FROM ROOTS AND LEAVES 1
- Subcellular Localization of the Starch Degradative and Biosynthetic Enzymes of Spinach Leaves 1
- Oxidative Turnover of Soybean Root Glutamine Synthetase. In Vitro and in Vivo Studies1
- Glutamine Synthetase of Pea Leaves: Divalent Cation Effects, Substrate Specificity, and Other Properties 1
