Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae.
AUTOR(ES)
Abraham, S N
RESUMO
Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-binding activity and trypsin sensitivity than native fimbriae. Removal of glycerol by dialysis results in spontaneous reassembly of the linear polymers into structures morphologically, antigenically, and functionally indistinguishable from native fimbriae.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=206335Documentos Relacionados
- Changes in macromolecular synthesis and nucleoside triphosphate levels during glycerol-induced growth stasis of Escherichia coli.
- Glycerol-induced development of catalytically active conformation of Crotalus adamanteus L-amino acid oxidase in vitro.
- Glomerular hemodynamics in established glycerol-induced acute renal failure in the rat.
- The curative effects of methylsulfonylmethane against glycerol-induced acute renal failure in rats
- Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae.