Glycogen depletion and acute branched-chain aminoacids supplementation on submaximal exercise in trained rats : implications on anaplerotic reactions / Depleção de glicogenio e suplementação aguda de aminoacidos de cadeia ramificada em ratos treinados : implicações sobre as reações anapleroticas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

During submaximal exercise, muscle glycogen utilization is important. Branched-chain AminoAcids supplementation has been utilized during the past years to provide energy for muscles in this condition, through their carbon skeletons, which might generate tricarboxilic acids cycle´s intermediates (TCAI). However, this hypothesis remains controversial. On the other hand, there is a recent discussion on the literature about the real importance of the rapid muscle TCAI raise and its role for de TCA cycle. This study aimed to evaluate if BCAA supplementation in trained rats submitted to sub-maximal exercise were capable of changing TCAI activity or transaminases (AAT and AST) concentration, or even performance in muscle depletion. Wistar Male Adult rats were trained for 8 weeks in a proper swimming system. In the last week they received BCAA supplementation. Before the final experiment, they trained for 1 hour, and then wento through a 24 h fasting. The final test consisted on swimming until exhaustion, then they were euthanized and tissues were kept in liquid Nitrogen until further analysis. BCAA group had higher muscle concentrations of malate. Isoleucine group had higher muscle transaminases? concentration, although rats supplemented with leucine alone had lower muscle glycogen levels than placebo group. We concluded that none of the BCAA supplementation was able to affect performance or TCA activity in our experimental conditions

ASSUNTO(S)

rat krebs cycle exercicios fisicos branched chain amino acids rato krebs aminoacidos de cadeia ramificada physical exercises ciclo de

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