Glycolate Pathway in Green Algae 1
AUTOR(ES)
Bruin, W. J.
RESUMO
By three criteria, the glycolate pathway of metabolism is present in unicellular green algae. Exogenous glycolate-1-14C was assimilated and metabolized to glycine-1-14C and serine-1-14C. During photosynthetic 14CO2 fixation the distributions of 14C in glycolate and glycine were similar enough to suggest a product-precursor relationship. Five enzymes associated with the glycolate pathway were present in algae grown on air. These were P-glycolate phosphatase, glycolate dehydrogenase (glycolate:dichloroindophenol oxidoreductase), l-glutamate:glyoxylate aminotransferase, serine hydroxymethylase, and glycerate dehydrogenase. Properties of glycerate dehydrogenase and the aminotransferase were similar to those from leaf peroxisomes. The specific activity of glycolate dehydrogenase and serine hydroxymethylase in algae was 1/5 to 1/10 that of the other enzymes, and both these enzymes appear ratelimiting for the glycolate pathway.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=396601Documentos Relacionados
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